FastQCFastQC Report
Thu 2 Jun 2016
DRR024850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4443353
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131180.29522750049343366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117870.26527264432963127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73640.16573069931648465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82250.053.8138281
GTATCAA148500.040.163631
ATCAACG186850.031.2002773
TCAACGC190000.030.6747234
TATCAAC193500.030.4688642
CAACGCA199550.029.4186865
GTGGTAT38750.029.2540171
TATAACG5150.029.213422
TGGTATC37650.028.2216972
AACGCAG210100.028.0737826
ACGCAGA235900.024.6835177
ACAACGC8050.024.5291963
CGCAGAG235500.024.5258858
GTACATG136700.023.2260531
GCAGAGT253900.022.433849
TACATGG134900.022.3052792
ACATGGG136000.021.3292793
ATAACGC6400.021.303353
TACCTGG32750.021.1030732
TAACGCA6550.020.8098664
Produced by FastQC (version 0.11.5)