FastQCFastQC Report
Thu 2 Jun 2016
DRR024849_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024849_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139750
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86860.27664622979536585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70040.22307508559598696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.14316426467075405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.052.4652521
GTATCAA106450.039.791051
ATCAACG126750.033.1177863
TCAACGC128600.032.6779174
TATCAAC131600.031.9355242
TACCTGG31750.031.2414472
CAACGCA135150.031.1637555
AACGCAG141250.030.0137716
GTACCTG35100.029.4660431
TATAACG3250.027.4829082
ACGCAGA156250.026.7409787
CGCAGAG155500.026.5975078
GTGGTAT28050.025.6427631
ATAACGC3500.025.517813
GTACATG81050.025.3474731
GTCTCGC8550.025.2928621
GCAGAGT164450.024.2354249
TCTGTCG10300.023.7276528
TACATGG83650.023.4348682
TGGTATC29250.023.1435012
Produced by FastQC (version 0.11.5)