FastQCFastQC Report
Thu 2 Jun 2016
DRR024849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139750
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97640.3109801735806991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82780.2636515646150171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58750.18711680866311012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.049.7340051
GTATCAA108950.039.7566341
TACCTGG33050.035.133612
GTACCTG35250.033.621621
ATCAACG138400.031.1480263
TCAACGC139850.030.7850844
TATCAAC142850.030.1777172
CAACGCA146450.029.5902655
AACGCAG152900.028.4627116
GTGGTAT31600.027.8311861
ACAACGC5850.027.3224933
GTACATG81350.026.1311241
ACGCAGA169800.025.3526677
TGGTATC33000.025.2148632
CGCAGAG171250.025.0007848
TACATGG81600.024.830442
TAACGCA4900.023.9801164
ACCTGGG46450.023.8849093
GCAGAGT180000.023.2893969
TATAACG3650.023.183392
Produced by FastQC (version 0.11.5)