FastQCFastQC Report
Thu 2 Jun 2016
DRR024848_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024848_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8008592
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141620.17683507912501972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112390.14033677830010569No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA84750.10582384519026565No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145900.045.2385751
GTATCAA244650.040.660021
TCAACGC321650.030.6894744
ATCAACG323250.030.5670363
TATCAAC335100.029.6267552
CAACGCA363550.027.1781335
AACGCAG374900.026.4036586
ACGCAGA386250.025.2257397
GTACATG364550.024.6563011
CGCAGAG399950.024.196678
TACATGG366600.022.9650672
GCAGAGT417050.022.573449
ACATGGG381450.021.4669193
GTGGTAT75100.020.28161
AGAGTAC381250.019.46602210-11
CAGAGTA404900.018.9674610-11
GAGTACA272650.018.8801631
CATGGGG282550.018.532914
GTACTTT203600.018.08568414-15
AGTACTT208800.018.08555612-13
Produced by FastQC (version 0.11.5)