FastQCFastQC Report
Thu 2 Jun 2016
DRR024848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8008592
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171010.21353316538038147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140680.1756613397211395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88490.11049382962698062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154000.045.940821
GTATCAA262850.041.1257321
ATCAACG356250.030.1440223
TCAACGC356150.030.1019764
TATCAAC370000.029.1512452
CAACGCA386350.027.7733125
AACGCAG396250.027.161266
ACGCAGA428800.024.802297
GTACATG367200.024.2120061
CGCAGAG440850.024.0177528
TACATGG371400.022.7241692
GCAGAGT458200.022.5544459
ACATGGG390650.021.0986213
AGAGTAC418850.020.32552710-11
AGTACTT237550.019.18027312-13
GTACTTT234400.018.91673914-15
GAGTACT237550.018.88351812-13
CAGAGTA448300.018.8225610-11
GTGGTAT81200.018.4801831
CATGGGG288900.018.1396964
Produced by FastQC (version 0.11.5)