FastQCFastQC Report
Thu 2 Jun 2016
DRR024847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041062
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81030.2005165968747819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70900.17544892901915388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41300.10220085710142533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95050.033.6308671
GTATCAA151850.032.2887151
ATCAACG170750.028.2174363
TCAACGC172550.027.9772194
CAACGCA178000.027.1206115
TATCAAC178650.027.0495872
AACGCAG184500.026.1628746
ACGCAGA201700.023.7451177
CGCAGAG200700.023.722338
GTACATG92850.022.9855691
GAGTACT129450.021.56569912-13
TACATGG93800.021.3991032
GCAGAGT216150.021.3091499
AGAGTAC167650.021.02582410-11
ACATGGG96400.020.0897523
TCTGTCG11550.019.9392768
CTGTCGC13150.019.3002669
AGTACTT139400.019.1666812-13
TATAACG4200.019.0269932
GTCTCGC11800.018.7239341
Produced by FastQC (version 0.11.5)