FastQCFastQC Report
Thu 2 Jun 2016
DRR024847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041062
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90650.22432222024804369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83810.20739597660219022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.1277386983916604No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103150.033.8881231
GTATCAA159350.030.793581
ATCAACG185500.026.1606523
TCAACGC186850.025.9845834
CAACGCA193550.025.255085
TATCAAC193300.025.2516082
AACGCAG199650.024.623486
CGCAGAG218700.022.1339728
ACGCAGA220150.022.1162767
GTACATG87500.021.9371411
GAGTACT141650.021.18435712-13
AGAGTAC180750.020.9309310-11
TACATGG89650.020.6666222
TACAACG4800.020.5731892
GCAGAGT235200.020.1416079
ACATGGG91150.019.449013
AGTACTT153050.019.25329812-13
GTACTTT162300.018.32973314-15
GTCGTAC1550.001027086518.2115731
GTACCGT2603.672085E-718.0764736
Produced by FastQC (version 0.11.5)