FastQCFastQC Report
Thu 2 Jun 2016
DRR024846_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024846_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716219
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129250.47584528346204774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.38678766329224556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72030.26518480284542595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40210.1480366642012297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35410.13036504052140124No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.10768645679895472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.050.6823921
GTATCAA122000.039.961641
TACCTGG27650.034.6838572
ATCAACG156100.030.7467523
TCAACGC159000.030.2445344
TATCAAC160700.030.0432532
TACAACG7200.030.0343132
GTGGTAT33100.029.685381
GTACCTG34100.029.2284531
CAACGCA167550.028.755695
AACGCAG173150.028.0679346
ACAACGC8050.028.0299133
TGGTATC34750.027.462092
TAACGCA3850.026.8615064
ACCTGGG36200.026.1014023
TATAACG3350.025.2592262
ACGCAGA192750.025.0426627
CGCAGAG193600.024.8351468
GTACAAC10750.024.0535161
GCAGAGT205200.023.0876459
Produced by FastQC (version 0.11.5)