FastQCFastQC Report
Thu 2 Jun 2016
DRR024846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716219
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143650.5288601545015332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122730.45184132796361415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93830.3454434270579802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40960.15079785540120294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35470.1305859358173991No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.12583668695344521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73200.051.6512261
GTATCAA129050.040.630531
TACCTGG26650.037.5749172
TACAACG5750.031.886912
GTACCTG33200.030.8783471
ACCTGGG32200.030.5133673
ATCAACG171950.030.4293483
TATAACG3400.030.4200152
TCAACGC173700.030.0078894
TATCAAC175450.029.8234232
CAACGCA180850.028.7695355
ACAACGC6600.028.4915283
TAACGCA4150.028.3136374
AACGCAG186600.028.0839986
GTGGTAT34050.025.5499631
ACGCAGA211950.024.6576187
CGCAGAG213700.024.2353258
ATAACGC4150.023.788713
TGGTATC35800.023.5063762
GCAGAGT223000.022.8031149
Produced by FastQC (version 0.11.5)