FastQCFastQC Report
Thu 2 Jun 2016
DRR024845_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024845_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378364
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106120.314116536879981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91370.2704563510622301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.16472470106832776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.046.7478561
GTATCAA125450.036.797811
ATCAACG150200.030.2951933
TCAACGC152250.029.9790134
TATCAAC158000.028.8591122
CAACGCA158600.028.689385
AACGCAG165050.027.736646
GTACATG110050.026.4412351
TACATGG111250.025.0989022
ACGCAGA183450.024.519117
CGCAGAG184800.024.1361648
ACATGGG113050.023.7013233
GCAGAGT197200.022.0226259
GTGGTAT39550.021.3947491
TACCTGG25150.021.307632
GAGTACT126250.020.88365712-13
AGAGTAC171550.020.67152410-11
CATGGGG64700.020.1974224
GTACCGT2109.536416E-720.1434426
GTACCTG28850.019.8790281
Produced by FastQC (version 0.11.5)