FastQCFastQC Report
Thu 2 Jun 2016
DRR024845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378364
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121560.3598191313902232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107460.3180829537610512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71670.2121441028853019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79200.046.3194731
GTATCAA136850.037.9762151
ATCAACG174650.029.5261733
TCAACGC176600.029.2486214
TATCAAC178100.029.1666662
CAACGCA182250.028.445035
AACGCAG188850.027.4994746
ACGCAGA213850.024.1744237
CGCAGAG216100.023.9444718
GTACATG109950.023.7406141
TACATGG109500.022.8823972
GCAGAGT226050.022.3499559
TATACCG2659.076757E-1021.2830475
AGAGTAC201350.020.98363510-11
GTACCGT2258.01283E-820.8879876
ACATGGG115800.020.8246613
TGGTATC43050.020.4200532
GTGGTAT44500.020.3981841
GAGTACT151950.020.2897112-13
ACAACGC5150.020.0809653
Produced by FastQC (version 0.11.5)