FastQCFastQC Report
Thu 2 Jun 2016
DRR024844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413303
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104630.30653592722357204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89420.2619749843480054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.15483536035330003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.052.3764081
GTATCAA125850.040.3794021
ATCAACG155650.032.584813
TCAACGC158050.032.0900044
TATCAAC161200.031.43472
CAACGCA164650.030.9178735
AACGCAG170700.029.9024896
ACAACGC6600.027.7756043
TATAACG3050.027.7413922
GTGGTAT32850.026.7604641
ACGCAGA191350.026.2333557
CGCAGAG191300.025.9699488
TGGTATC32700.025.156252
GTACATG105000.023.8630141
GCAGAGT203600.023.685059
TACATGG105200.022.5647872
ACATGGG104700.021.7290923
GTACCGG3500.021.4901471
GAGTACT142150.020.97815112-13
AGAGTAC187000.020.72305310-11
Produced by FastQC (version 0.11.5)