FastQCFastQC Report
Thu 2 Jun 2016
DRR024844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413303
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114420.33521782273651063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103120.3021120597849063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66620.19517751573768868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.052.103681
GTATCAA126600.039.1976131
GTGGTAT32750.031.5974661
TGGTATC32850.031.2011812
ATCAACG163100.030.2969343
TCAACGC164700.029.8502524
TATCAAC169550.029.2275712
CAACGCA171650.028.7237825
AACGCAG180400.027.407946
ACAACGC7750.026.0865523
ACGCAGA201400.024.4083047
CGCAGAG200550.024.3242848
GTACATG102950.023.9411951
ATAACGC3750.023.8216723
TACATGG99900.022.6846182
GCAGAGT214200.022.3353989
TACAACG9450.021.8912582
ACATGGG102650.021.2524473
TACCTGG28900.021.1492712
AGAGTAC202200.020.90669610-11
Produced by FastQC (version 0.11.5)