FastQCFastQC Report
Thu 2 Jun 2016
DRR024843_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024843_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7319774
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141180.19287480733694784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108510.1482422817972249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114900.052.494621
GTATCAA218650.043.367521
ATCAACG283250.033.1735343
TCAACGC285500.032.9283334
TATCAAC302950.031.1262342
CAACGCA316400.029.6830025
AACGCAG326500.028.8200076
ACGCAGA350650.026.5394977
GTACATG348600.025.5153751
CGCAGAG364050.025.4459158
TACATGG338600.025.2113152
GTGGTAT48550.024.8858281
GCAGAGT380100.023.7902879
ACATGGG360700.022.9228463
TGGTATC54850.020.9112432
CATGGGG233300.020.6315144
AGAGTAC365550.020.03791210-11
CAGAGTA379000.019.258610-11
GTACTTT192250.018.94567114-15
AGTACTT197300.018.53170212-13
Produced by FastQC (version 0.11.5)