FastQCFastQC Report
Thu 2 Jun 2016
DRR024843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7319774
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160290.21898217076100984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127700.1744589382131197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92950.12698479488574374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119450.054.613631
GTATCAA233400.044.2126431
ATCAACG302550.033.6872063
TCAACGC307100.033.179264
TATCAAC320500.031.8896122
CAACGCA331400.030.7889215
AACGCAG342650.029.7907496
ACGCAGA384000.026.3616147
GTGGTAT50300.025.433821
CGCAGAG398400.025.3498048
GTACATG350150.024.044161
GCAGAGT412400.024.0106229
TACATGG337650.023.4612352
TGGTATC55100.021.6720452
ACATGGG362550.021.3304923
AGAGTAC395000.019.92846110-11
CAGAGTA408000.019.489310-11
AGTACTT221750.019.34914212-13
GTACTTT217750.018.72258814-15
CATGGGG237700.018.4080474
Produced by FastQC (version 0.11.5)