FastQCFastQC Report
Thu 2 Jun 2016
DRR024842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12583320
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457010.36318713980094286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343380.27288505736165014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211500.16807964829631608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210850.059.31281
GTATCAA432450.045.248751
ATCAACG527650.036.678053
TCAACGC530900.036.4606364
TATCAAC551950.035.2542
CAACGCA559950.034.5440255
AACGCAG591250.032.90356
CGCAGAG645450.029.6876078
ACGCAGA652600.029.6014217
GTACATG395800.027.2613451
GCAGAGT702550.026.7997539
TACATGG390200.026.3857612
ACATGGG397000.025.2327353
GTGGTAT80250.025.1920681
TGGTATC84750.022.2997042
TACAACG26950.022.1542852
AGAGTAC667550.021.83299810-11
CAGAGTA701100.021.5222710-11
GAGTACT507750.021.27978312-13
ACAACGC31000.020.7744833
Produced by FastQC (version 0.11.5)