FastQCFastQC Report
Thu 2 Jun 2016
DRR024842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12583320
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516870.41075805113435887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398200.31645066643779224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255090.2027207446047625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227950.060.1087071
GTATCAA455300.044.278311
ATCAACG564800.035.2942473
TCAACGC568550.034.975494
CAACGCA588900.033.846695
TATCAAC591650.033.835572
AACGCAG617350.032.3245776
ACAACGC21550.029.015973
ACGCAGA691000.028.6666057
CGCAGAG688700.028.6325728
GCAGAGT748600.025.858129
GTACATG398300.025.6144921
TACATGG393950.024.823042
ACATGGG396100.023.821843
GTGGTAT87650.023.7732371
TACAACG26600.022.4468212
TGGTATC89550.022.10292
AGAGTAC717200.021.7055410-11
CAGAGTA748850.021.34041810-11
GAGTACT551900.021.29224412-13
Produced by FastQC (version 0.11.5)