FastQCFastQC Report
Thu 2 Jun 2016
DRR024841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10511482
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482710.45922163972691954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331380.31525526086616523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209570.19937245766106054No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202800.060.504781
GTATCAA435350.048.918911
ATCAACG550350.038.289233
TCAACGC550650.038.2153324
TATCAAC575300.036.8197562
CAACGCA585400.035.9070245
AACGCAG606400.034.7237366
ACGCAGA660250.031.5713257
CGCAGAG662200.031.2926258
GCAGAGT714150.028.5226969
GTACATG507850.028.1512741
TACATGG495050.027.8703962
ACATGGG514300.025.9292353
GTGGTAT75800.023.1963421
CATGGGA233100.023.1688234
AGAGTAC677450.022.44326610-11
CAGAGTA703800.022.297510-11
GAGTACT465550.022.09826312-13
TGGTATC77850.021.7988012
CATGGGG242500.021.7086334
Produced by FastQC (version 0.11.5)