FastQCFastQC Report
Thu 2 Jun 2016
DRR024841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10511482
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT526420.5008047390463115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385620.3668559771115053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247030.23500967798831793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225600.060.255921
GTATCAA461800.046.487121
ATCAACG589400.036.111623
TCAACGC589900.035.9716764
TATCAAC613900.034.770012
CAACGCA613750.034.596815
AACGCAG635650.033.4935726
ACGCAGA712500.029.62897
CGCAGAG714150.029.3497248
GTACATG510450.027.902531
TACATGG499850.027.7196082
GCAGAGT767850.026.8870669
ACATGGG518850.025.7621253
CATGGGG244100.022.9311224
TGGTATC83400.022.4933152
GTGGTAT87500.022.3090461
GAGTACT509750.021.79920612-13
CAGAGTA760950.021.7238410-11
AGAGTAC733400.021.68122910-11
CATGGGA229700.021.6678734
Produced by FastQC (version 0.11.5)