FastQCFastQC Report
Thu 2 Jun 2016
DRR024840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13185858
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445850.33812740892553217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324020.2457329663340831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209950.1592236166960087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204450.056.4555851
GTATCAA413350.045.155961
ATCAACG505850.036.427093
TCAACGC505600.036.4154134
CAACGCA531400.034.6830565
TATCAAC535350.034.5112762
AACGCAG555500.033.286846
ACGCAGA619650.029.5980517
CGCAGAG626900.029.0816528
GTACATG412850.027.0011221
GCAGAGT680050.026.3386849
TACATGG407800.026.030532
ACATGGG418400.024.2673443
GTGGTAT86600.021.770531
AGAGTAC644900.021.38275510-11
CAGAGTA668700.021.27888110-11
GAGTACT473600.020.77490612-13
CATGGGG254950.020.5938194
TACCTGG100450.020.5456942
AGTACTT483500.019.8586512-13
Produced by FastQC (version 0.11.5)