FastQCFastQC Report
Thu 2 Jun 2016
DRR024840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13185858
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT499280.3786480940413586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373070.2829319108396284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246830.1871929759898825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211150.058.274951
GTATCAA431800.045.544171
ATCAACG535700.036.2906463
TCAACGC537500.036.0866784
CAACGCA556400.034.9284485
TATCAAC567750.034.449032
AACGCAG578650.033.5853966
ACGCAGA658100.029.3649397
CGCAGAG664900.028.9227098
GCAGAGT718750.026.33739
GTACATG413300.026.2439461
TACATGG407250.025.2365462
ACAACGC22750.023.9727253
ACATGGG419800.023.5413363
AGAGTAC685250.021.49019210-11
CAGAGTA710800.021.33260510-11
GTGGTAT86800.021.0797351
GAGTACT515950.021.04088412-13
TACCTGG96950.020.8041652
CATGGGG254350.020.5477354
Produced by FastQC (version 0.11.5)