FastQCFastQC Report
Thu 2 Jun 2016
DRR024839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8712676
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256210.29406579562926477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202440.23235111692435254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117200.13451665137094504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130050.060.694921
GTATCAA259850.044.090481
ATCAACG310000.036.421563
TCAACGC312250.036.113124
TATCAAC329550.034.563422
CAACGCA328800.034.337685
AACGCAG344400.032.849236
CGCAGAG384050.029.151368
ACGCAGA387600.028.9947
GCAGAGT424450.025.9115949
GTACATG228350.024.8907131
TACATGG228850.023.8282242
GTGGTAT53350.023.1757261
ACATGGG231550.022.3912283
CAGAGTA419250.021.94476910-11
GAGTACT317400.021.51486412-13
AGAGTAC404050.021.00219510-11
TGGTATC55350.020.8930632
GTACTTT355350.019.13097214-15
AGTACTT330100.019.11387312-13
Produced by FastQC (version 0.11.5)