FastQCFastQC Report
Thu 2 Jun 2016
DRR024839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8712676
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295120.3387248647832193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245790.28210620938962955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149060.17108406188867806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142400.058.8944241
GTATCAA275900.043.4220241
ATCAACG339150.034.906173
TCAACGC340250.034.601774
CAACGCA352500.033.5059625
TATCAAC360000.033.0542952
AACGCAG372000.031.9010286
ACGCAGA421250.027.9802387
CGCAGAG422400.027.6921888
GTGGTAT55200.026.5857161
GTACATG222550.025.9328211
GCAGAGT461000.024.8331979
TACATGG223550.024.3961682
TGGTATC57550.023.6914142
TATAACG9550.023.6306782
ACATGGG228700.023.003943
AGAGTAC438750.021.0207810-11
CAGAGTA458700.020.90567410-11
ACAACGC13050.020.8956453
GAGTACT356650.020.4901212-13
Produced by FastQC (version 0.11.5)