FastQCFastQC Report
Thu 2 Jun 2016
DRR024838_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024838_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7652738
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147160.19229718827431436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.13985844020793603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116350.055.7214661
GTATCAA236050.045.9481741
ATCAACG294000.036.455813
TCAACGC295800.036.215244
CAACGCA316750.033.834785
TATCAAC319500.033.695312
AACGCAG333550.032.129156
ACGCAGA364350.028.8455187
CGCAGAG363000.028.7313568
GTACATG334500.025.8610611
TACATGG324550.025.6387252
GCAGAGT399350.025.4568989
ACATGGG334850.024.1747553
GTGGTAT49600.022.5590041
AGAGTAC371900.020.97898710-11
CATGGGG190300.020.8225524
CAGAGTA392750.020.16444810-11
GAGTACT201050.019.84641612-13
AGTACTT207000.019.3554212-13
TGGTATC54900.018.581992
Produced by FastQC (version 0.11.5)