FastQCFastQC Report
Thu 2 Jun 2016
DRR024838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7652738
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160280.20944137902016244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119830.15658447995998295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121750.055.9064561
GTATCAA240000.043.9427071
ATCAACG294550.035.3072783
TCAACGC299750.034.63644
CAACGCA314750.033.0156025
TATCAAC320500.032.5512282
AACGCAG332200.031.2952826
ACGCAGA379200.027.1173237
CGCAGAG378450.026.9223468
GTACATG335350.025.0241261
TACATGG331250.024.4549922
GCAGAGT418500.023.6721299
ACATGGG345250.022.5645643
GTGGTAT54300.021.5705871
GAGTACT216550.019.6296512-13
TGGTATC55400.019.6038022
AGAGTAC388750.019.59028610-11
CAGAGTA410350.019.46958210-11
AGTACTT217600.019.13537612-13
GTACTTT220050.018.83689514-15
Produced by FastQC (version 0.11.5)