FastQCFastQC Report
Thu 2 Jun 2016
DRR024837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5901302
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313320.530933682092528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235530.39911531387480254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135220.22913587543901326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80640.13664781094070427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146000.061.7021261
GTATCAA304100.047.5738751
ATCAACG385250.037.313963
TCAACGC388550.036.945844
TATCAAC408900.035.296442
CAACGCA408550.035.1369065
AACGCAG426700.033.794556
CGCAGAG460600.030.6727878
ACGCAGA465650.030.5942827
GCAGAGT491100.027.9735189
TATAACG6000.026.640192
GTACATG362650.026.3447151
TACATGG357100.026.0271422
GTGGTAT50400.025.0012511
ACATGGG364800.024.548033
TGGTATC52050.023.3028282
TACCTGG38950.023.174132
CAGAGTA488350.022.33737610-11
CATGGGA190400.022.2187184
CATGGGG137700.021.8127444
Produced by FastQC (version 0.11.5)