FastQCFastQC Report
Thu 2 Jun 2016
DRR024837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5901302
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357330.605510444983158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277920.4709469198492129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161760.27410900170843655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64570.10941653214832929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154950.062.9305951
GTATCAA318100.046.033031
ATCAACG407800.035.7394373
TCAACGC411900.035.2464834
CAACGCA427300.033.9981885
TATCAAC433100.033.69512
AACGCAG447900.032.549686
GTGGTAT51550.031.1157191
CGCAGAG497500.028.7832348
ACGCAGA502150.028.742047
TGGTATC54100.028.3304542
TATAACG4400.026.712872
GCAGAGT530100.026.516669
TACAACG12500.025.9520872
TACCTGG40850.025.8954622
GTACATG358250.025.682471
TACATGG348550.025.655392
ACATGGG358750.023.9299663
CAGAGTA526650.021.86718410-11
CATGGGA182100.021.8348454
Produced by FastQC (version 0.11.5)