FastQCFastQC Report
Thu 2 Jun 2016
DRR024836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7956651
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191740.24098078450342988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146380.183971874598999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80830.10158796709821757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122750.047.4549031
GTATCAA225500.041.0516361
ATCAACG264350.034.4450653
TCAACGC265050.034.3523674
CAACGCA278450.032.8013235
TATCAAC283600.032.125422
AACGCAG287600.031.9676176
ACGCAGA320950.028.3383057
CGCAGAG323750.027.8450058
GTACATG200600.025.1712421
GCAGAGT357500.024.7692769
TACATGG200600.023.2009352
ACATGGG205850.021.740163
AGAGTAC318700.021.03032310-11
ACAACGC17250.020.9835193
GAGTACT238400.020.7588512-13
CAGAGTA351250.020.1452610-11
GTACCGG7100.019.8652571
TACCTGG60000.019.352942
AGTACTT253950.018.66417712-13
Produced by FastQC (version 0.11.5)