FastQCFastQC Report
Thu 2 Jun 2016
DRR024836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7956651
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218030.274022324216558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173110.2175664107926815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99200.12467557016136563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125900.049.3498231
GTATCAA232250.040.7658581
ATCAACG278300.033.413943
TCAACGC278550.033.3074384
CAACGCA289200.032.1296235
TATCAAC301600.031.03522
AACGCAG301250.030.9062616
ACGCAGA343400.026.878717
CGCAGAG347700.026.3026758
ACAACGC12750.025.4421353
GTACATG197650.024.3913351
GCAGAGT380250.023.630919
TACATGG196150.023.4883442
ACATGGG200850.021.8151743
AGAGTAC341550.020.97659110-11
GAGTACT261400.020.45966112-13
TATAACG6550.020.0970062
TACCTGG59200.019.9327242
TACAACG16550.019.6004352
CAGAGTA376650.019.54583510-11
Produced by FastQC (version 0.11.5)