FastQCFastQC Report
Thu 2 Jun 2016
DRR024835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8790851
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202490.23034174962128243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160110.1821325375666133No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127200.055.034111
GTATCAA246050.044.195361
TCAACGC292000.036.7036824
ATCAACG292250.036.6893
CAACGCA308750.034.7124755
TATCAAC313250.034.441842
AACGCAG324350.033.229076
CGCAGAG357400.029.681468
ACGCAGA361800.029.6076417
GCAGAGT397000.026.3182649
GTACATG231650.025.348631
TACATGG223800.024.7445722
ACATGGG233050.023.1556973
GAGTACT269850.021.77934612-13
AGAGTAC363350.021.55694410-11
CAGAGTA390700.021.46142210-11
GTGGTAT56800.021.2720411
TGGTATC59250.019.5182782
GTACTTT302000.019.36702314-15
AGTACTT286700.019.19608912-13
Produced by FastQC (version 0.11.5)