FastQCFastQC Report
Thu 2 Jun 2016
DRR024835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8790851
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205620.23390226952999202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165520.18828666303182706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95240.10833990929888357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124750.054.676171
GTATCAA237550.042.951191
TCAACGC279050.035.8588264
ATCAACG281150.035.6371883
CAACGCA291850.034.334445
TATCAAC303350.033.246152
AACGCAG309400.032.5080536
ACGCAGA351000.028.4122817
CGCAGAG349250.028.298338
GCAGAGT387900.025.006219
GTACATG228300.024.6019251
TACATGG222300.023.3288962
ACATGGG228100.021.9318083
GAGTACT270700.020.96316512-13
CAGAGTA381650.020.80419310-11
AGAGTAC357700.020.6599710-11
GTGGTAT56850.019.693241
AGTACTT286000.018.74897812-13
GTACTTT303100.018.57514-15
CATGGGG135650.017.6360134
Produced by FastQC (version 0.11.5)