FastQCFastQC Report
Thu 2 Jun 2016
DRR024833_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024833_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10160806
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293740.28909123941545584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215020.21161707053554607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129570.12751941135378433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148650.054.5903661
GTATCAA298450.045.2274131
ATCAACG357450.037.32273
TCAACGC358800.037.1407784
CAACGCA376650.035.3679665
TATCAAC378900.035.2255172
AACGCAG393050.033.9139866
ACGCAGA438500.030.1523067
CGCAGAG440100.029.720978
GTACATG298750.026.8951681
GCAGAGT487200.026.3557039
TACATGG292650.026.2173162
ACATGGG301150.024.3354423
GTGGTAT65150.022.3711031
GAGTACT323500.021.16722912-13
AGAGTAC450500.021.14755210-11
CAGAGTA478650.020.9250510-11
CATGGGG190600.020.7900244
TGGTATC69350.019.9982592
AGTACTT336300.019.41826412-13
Produced by FastQC (version 0.11.5)