FastQCFastQC Report
Thu 2 Jun 2016
DRR024832_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024832_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10205730
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287020.2812341694322699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208680.20447336937191163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129310.12670333234369321No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138800.056.669011
GTATCAA279800.044.9316981
ATCAACG332700.037.1042443
TCAACGC335500.036.779314
TATCAAC357550.034.7382132
CAACGCA355450.034.689095
AACGCAG370900.033.418436
ACGCAGA415600.029.6431397
CGCAGAG414750.029.4869778
GTACATG262550.027.2547231
GCAGAGT459600.026.108399
TACATGG266000.025.5563092
ACATGGG270100.024.2268143
AGAGTAC429000.021.55034610-11
CAGAGTA452050.021.07013510-11
GTGGTAT57550.020.8321441
GAGTACT331300.020.69718412-13
CATGGGG173050.020.264414
AGTACTT345950.019.41995612-13
GTACTTT371350.018.23692114-15
Produced by FastQC (version 0.11.5)