FastQCFastQC Report
Thu 2 Jun 2016
DRR024832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10205730
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320110.3136571318269247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239540.23471128473906328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152900.14981779843284115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150150.058.0211331
GTATCAA302150.044.9930881
ATCAACG371250.036.0823063
TCAACGC370550.035.896014
CAACGCA385850.034.5579035
TATCAAC393250.034.1234972
AACGCAG402450.033.2372636
ACGCAGA458300.028.9894497
CGCAGAG459000.028.7912318
GTACATG265950.025.877061
GCAGAGT501950.025.737839
TACATGG263950.024.5112292
ACATGGG267950.023.1451153
AGAGTAC474650.021.68344310-11
CAGAGTA495900.021.03384610-11
GAGTACT373550.020.9156112-13
ACAACGC16800.020.7105083
GTGGTAT63650.020.32381
AGTACTT388950.019.9424912-13
ATAACGC10500.019.2551753
Produced by FastQC (version 0.11.5)