FastQCFastQC Report
Thu 2 Jun 2016
DRR024831_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024831_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8195335
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280720.342536333170029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215840.26336934365709275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127450.15551530230307853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135450.058.5911561
GTATCAA265200.043.7177931
ATCAACG325500.035.1071663
TCAACGC326500.034.9970784
TATCAAC338000.033.8820842
CAACGCA340350.033.6164055
AACGCAG358350.032.1101236
ACGCAGA398800.028.60577
CGCAGAG397200.028.4236958
GTACATG205600.026.5032391
GCAGAGT426850.025.7003889
ACAACGC15700.025.15023
TACATGG209800.024.7384852
TATAACG7750.024.2643012
TACAACG15850.024.02512
ACATGGG208900.023.7622343
GTGGTAT55150.023.5289331
TACCTGG60250.023.1744232
ATAACGC8450.022.808093
TGGTATC56000.022.330742
Produced by FastQC (version 0.11.5)