FastQCFastQC Report
Thu 2 Jun 2016
DRR024831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8195335
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324650.39613999915805764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256010.31238503368074644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160270.19556247548147818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144450.058.9729961
GTATCAA290400.044.3819351
ATCAACG356800.035.6706923
TCAACGC358950.035.325574
CAACGCA372050.034.119645
TATCAAC374900.034.0613942
AACGCAG389950.032.673766
ACAACGC13600.030.7679943
ATAACGC7500.029.4635263
CGCAGAG436550.028.6138068
ACGCAGA441550.028.6090897
TACAACG16050.026.3642622
GCAGAGT470550.025.9879
GTACATG213300.025.6472681
GTGGTAT59100.024.912031
TATAACG7900.024.4008462
TACATGG216500.024.1270542
ACATGGG212450.023.3919473
TGGTATC60750.022.9857272
AGAGTAC441850.022.02191210-11
Produced by FastQC (version 0.11.5)