FastQCFastQC Report
Thu 2 Jun 2016
DRR024830_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024830_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6967915
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110990.159287247332954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81490.11695033593262835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119200.044.8082121
GTATCAA215700.039.540551
ATCAACG252250.033.395473
TCAACGC252750.033.3832864
CAACGCA267100.031.7140925
TATCAAC267250.031.559882
AACGCAG278250.030.4568616
ACGCAGA310050.026.9692487
CGCAGAG307850.026.8247348
GTACATG245250.024.4047831
TACATGG241850.023.8522342
GCAGAGT340750.023.476139
ACATGGG249400.022.279313
AGAGTAC295050.019.9278210-11
GAGTACT171450.019.69501312-13
TCTGTCG19150.019.6346878
CAGAGTA334700.018.76069310-11
CATGGGG135750.018.0060844
AGTACTT183450.017.83029212-13
GTACTTT190000.017.6234314-15
Produced by FastQC (version 0.11.5)