FastQCFastQC Report
Thu 2 Jun 2016
DRR024830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6967915
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121340.17414104506154282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94240.13524849255480298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119450.046.742321
GTATCAA220450.041.5433541
ATCAACG265450.033.9698223
TCAACGC267200.033.631154
CAACGCA277350.032.48515
TATCAAC282600.032.024772
AACGCAG292250.030.8125866
ACGCAGA330150.027.0176247
CGCAGAG327300.026.9938458
GTACATG239200.024.5807171
TACATGG233950.023.8335672
GCAGAGT363300.023.7368769
ACATGGG244250.022.2510593
AGAGTAC314300.020.61924410-11
GAGTACT196550.020.33546612-13
AGTACTT207850.018.98119512-13
CAGAGTA357400.018.7572910-11
GTACTTT212050.018.57199914-15
TCTGTCG22250.017.9531978
CATGGGG140150.017.5052224
Produced by FastQC (version 0.11.5)