FastQCFastQC Report
Thu 2 Jun 2016
DRR024829_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024829_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9555104
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277770.2907032723034726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213580.22352451632132944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121850.1275234680857477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149400.056.989951
GTATCAA291800.044.8553621
ATCAACG355000.036.242093
TCAACGC355900.036.109684
CAACGCA376350.034.185215
TATCAAC377700.034.1658062
AACGCAG395350.032.6820876
ACGCAGA437950.029.2453277
CGCAGAG437700.029.0145918
GTACATG307300.026.176881
GCAGAGT476800.026.1127979
TACATGG298100.025.5633262
ACATGGG307600.024.0844463
GTGGTAT63200.023.8047331
TGGTATC65600.021.2837892
CAGAGTA472900.021.08040810-11
GAGTACT317700.020.93237312-13
AGAGTAC451200.020.9119310-11
CATGGGG179500.020.1117524
AGTACTT328500.019.43584812-13
Produced by FastQC (version 0.11.5)