FastQCFastQC Report
Thu 2 Jun 2016
DRR024829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9555104
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324420.3395253468722057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252390.26414155199148015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145390.15215951600317484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161500.058.7216721
GTATCAA318000.044.911241
ATCAACG386550.036.284493
TCAACGC387200.036.1484764
CAACGCA403750.034.7598535
TATCAAC410500.034.3393362
AACGCAG423150.033.110526
ACGCAGA475550.029.1343067
CGCAGAG473600.029.0748838
GCAGAGT513200.026.2453029
GTACATG307750.025.6645131
TACATGG299700.025.1019042
GTGGTAT64650.023.356991
ACATGGG309500.023.2892193
TGGTATC64250.022.466672
GAGTACT351950.021.83226812-13
CAGAGTA507400.021.83087510-11
AGAGTAC486100.021.03755610-11
GTACTTT385250.019.75605814-15
AGTACTT364650.019.53822112-13
Produced by FastQC (version 0.11.5)