FastQCFastQC Report
Thu 2 Jun 2016
DRR024828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611903
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217580.28584179278164734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162590.21359967408938343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92980.12215079461732499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108750.053.6491281
GTATCAA211100.044.1180571
TCAACGC253350.036.103864
ATCAACG256550.035.6918143
CAACGCA266650.034.3218235
TATCAAC270450.033.9128882
AACGCAG275100.033.402096
ACGCAGA310900.029.1778987
CGCAGAG322100.027.870578
GTACATG241350.025.6736891
GCAGAGT357200.024.35549
TACATGG243500.024.0940672
GTGGTAT45450.023.8945161
ACATGGG243750.023.1413293
TACCTGG52500.020.5950572
AGAGTAC319750.020.59339110-11
CAGAGTA335150.020.55867210-11
GAGTACT217100.019.87259712-13
CATGGGG176150.019.4792354
TACAACG14350.019.328342
Produced by FastQC (version 0.11.5)