FastQCFastQC Report
Thu 2 Jun 2016
DRR024828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611903
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244510.3212205935887517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190170.24983240064935142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117900.15488899425018948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115650.054.4175871
GTATCAA219250.042.605771
TCAACGC267050.034.511644
ATCAACG268850.034.2750323
CAACGCA276350.033.384235
TATCAAC284250.032.5338672
AACGCAG285250.032.3753626
ACGCAGA327950.027.7146557
CGCAGAG341550.026.4458088
GTACATG240850.026.325221
TACATGG239700.025.145012
ACATGGG242450.024.0259723
GCAGAGT373700.023.5418479
GTGGTAT46900.022.966321
ACAACGC13250.021.2895873
TACCTGG54600.020.6657142
CATGGGG171350.020.598544
AGAGTAC338900.020.4193310-11
CAGAGTA356500.020.20220210-11
TATAACG6200.019.7157572
Produced by FastQC (version 0.11.5)