FastQCFastQC Report
Thu 2 Jun 2016
DRR024827_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024827_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11830591
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307490.25991093766997775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238790.20184114217117302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137200.11597053773560424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172550.054.9591671
GTATCAA329750.042.6034741
TCAACGC389300.035.4510464
ATCAACG390150.035.374263
CAACGCA412750.033.434955
TATCAAC422600.032.894922
AACGCAG431050.032.1546636
ACGCAGA480800.028.5437857
CGCAGAG482200.028.235548
GTACATG312250.025.883481
GCAGAGT536100.024.9057859
TACATGG307550.024.6408842
ACATGGG309250.023.6821253
GAGTACT355800.021.51731912-13
AGAGTAC488450.020.86960610-11
CAGAGTA528050.020.72411510-11
AGTACTT387750.018.82316412-13
CATGGGG179300.018.7449024
GTACTTT408200.018.69196714-15
GTGGTAT78650.018.352231
Produced by FastQC (version 0.11.5)