FastQCFastQC Report
Thu 2 Jun 2016
DRR024827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11830591
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342610.2895966904781004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271370.2293799185518289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163500.13820104168929515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179950.054.5309221
GTATCAA342500.042.660041
ATCAACG419150.034.258243
TCAACGC421550.034.017944
CAACGCA436500.032.928215
TATCAAC447150.032.2919242
AACGCAG454550.031.7129046
CGCAGAG513250.027.7540288
ACGCAGA517000.027.6799817
GTACATG306200.025.1338181
GCAGAGT569850.024.4613069
TACATGG302300.024.1390882
ACATGGG302250.022.8362733
GAGTACT394150.020.99150312-13
AGAGTAC520000.020.93620710-11
GTGGTAT84900.020.6117841
CAGAGTA560300.020.39914510-11
TGGTATC83200.019.2707392
AGTACTT419850.019.18605612-13
CATGGGG177100.018.5247254
Produced by FastQC (version 0.11.5)