FastQCFastQC Report
Thu 2 Jun 2016
DRR024826_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024826_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11194907
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299380.2674251782529323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226610.20242240511689824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134380.12003672741542203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164700.056.6673471
GTATCAA325250.044.538991
ATCAACG395800.036.152943
TCAACGC396550.036.0002944
CAACGCA417300.034.2412455
TATCAAC421150.034.169922
AACGCAG432100.033.185576
ACGCAGA482050.029.3854797
CGCAGAG482550.029.1302438
GCAGAGT532950.025.8196749
GTACATG307800.025.158861
TACATGG307300.024.0648732
ACATGGG314700.022.6303163
AGAGTAC493700.021.480210-11
CAGAGTA523000.021.14396310-11
GAGTACT363000.020.92188512-13
GTGGTAT71150.020.3536241
CATGGGG184300.019.1291284
AGTACTT383800.019.06556912-13
GTACTTT402150.018.61064114-15
Produced by FastQC (version 0.11.5)