FastQCFastQC Report
Thu 2 Jun 2016
DRR024826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11194907
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334370.29868046246386865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265320.23700062894671656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162410.1450748987910306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169800.056.594321
GTATCAA330150.043.9669951
ATCAACG407950.035.2897263
TCAACGC410150.035.0542954
CAACGCA426400.033.8286135
TATCAAC430000.033.5238382
AACGCAG444250.032.4899566
ACGCAGA504100.028.3882267
CGCAGAG506750.028.0169418
GTACATG308450.025.1007821
GCAGAGT554500.025.0449229
TACATGG307250.024.3766462
ACATGGG310700.023.2282833
AGAGTAC521200.021.28449610-11
GTGGTAT75900.021.132691
ACAACGC20150.020.9999123
GAGTACT389000.020.82214512-13
CAGAGTA544550.020.65231310-11
AGTACTT405450.019.71654312-13
CATGGGG186400.019.2894574
Produced by FastQC (version 0.11.5)