FastQCFastQC Report
Thu 2 Jun 2016
DRR024825_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024825_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11451427
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367120.3205888663482726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280870.24527074224024656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164740.1438598001803618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222650.046.0563471
GTATCAA399100.040.1960871
ATCAACG470500.033.7202643
TCAACGC472450.033.6308364
TATCAAC494550.032.244572
CAACGCA494400.032.192665
ACAACGC25550.031.461693
AACGCAG516900.030.8973756
TACAACG27800.027.733842
ACGCAGA578400.027.4007327
CGCAGAG575100.027.335638
GTACATG306350.027.1037461
TACCTGG92050.026.4555592
TATAACG11850.026.1840322
TACATGG303300.025.6374072
GCAGAGT619250.024.7112489
ACATGGG307400.024.2230113
ATAACGC13550.023.5910073
GTACCTG111450.022.3589951
TCTGTCG27950.021.859388
Produced by FastQC (version 0.11.5)