FastQCFastQC Report
Thu 2 Jun 2016
DRR024825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11451427
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414980.36238278425911463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334190.29183262487723144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203150.1774014714498027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239350.047.7173921
GTATCAA424650.039.3573231
ATCAACG510200.032.3644873
TCAACGC513100.032.12554
CAACGCA530850.031.1221565
TATCAAC536800.030.8658352
AACGCAG556650.029.78016
TATAACG12100.029.1427522
TACCTGG97550.027.135372
ACAACGC21150.027.1209033
ACGCAGA628100.026.1587587
CGCAGAG626350.026.0891678
TACAACG23900.024.9839132
GTACATG296350.024.7458651
GCAGAGT671600.023.8065369
TACATGG296550.023.6075762
ATAACGC14550.023.2661293
GTACCTG116700.023.257681
ACATGGG302150.022.065223
AGAGTAC586650.021.43776910-11
Produced by FastQC (version 0.11.5)