FastQCFastQC Report
Thu 2 Jun 2016
DRR024824_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024824_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9590807
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385470.40191612655744197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297880.3105890880715252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177280.18484367373882093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107620.1122116209824679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183400.055.993851
GTATCAA354950.044.0994951
ATCAACG437350.035.5350153
TCAACGC440050.035.28424
TATCAAC452550.034.406862
CAACGCA464950.033.3815165
AACGCAG485700.032.0007826
ACGCAGA536450.028.631717
CGCAGAG532100.028.528798
GCAGAGT572200.025.822999
GTACATG378000.025.7741831
TACATGG375900.024.9262122
GTGGTAT72950.024.8154641
ACATGGG384000.023.3821773
GAGTACT359350.022.16786612-13
CAGAGTA555000.021.53402510-11
TGGTATC82450.021.439692
AGAGTAC532750.021.05720910-11
CATGGGG213300.020.7603074
GTACTTT392950.020.17673114-15
Produced by FastQC (version 0.11.5)