FastQCFastQC Report
Thu 2 Jun 2016
DRR024824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9590807
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451460.4707215982972027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352250.3672787910339558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220310.22970955415951966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194350.057.9186171
GTATCAA377950.044.232671
ATCAACG473150.035.0273553
TCAACGC477000.034.723924
TATCAAC485750.034.254282
CAACGCA493650.033.5432135
AACGCAG513700.032.279256
ACGCAGA584500.028.206267
CGCAGAG586550.027.8751748
GTACATG380600.026.028531
GCAGAGT626200.025.637349
GTGGTAT75150.025.600851
TACATGG381400.025.233892
ACATGGG392800.023.5679243
TGGTATC80100.022.7156682
GAGTACT405850.021.55587612-13
CAGAGTA613000.021.37088810-11
AGAGTAC588450.021.16832210-11
CATGGGG223300.020.8591274
AGTACTT416850.020.41770212-13
Produced by FastQC (version 0.11.5)